منابع مشابه
The Chemical Labeling of Glutamate Decarboxylase in
Mouse brain glutamate decarboxylase(s) was specifically titrated in vivo and in crude brain homogenates by a combination of gabaculine and [a-'H]acetylenic yaminobutyric acid. This specific titration is based on the differential spectra of action of these two mechanism-based enzyme inactivators. The specificity of the titration in vitro was demonstrated by showing that the time course of radioa...
متن کاملChemical labeling of protein in living cells.
Fluorescence imaging of fluorophore-labeled proteins opens a window into cellular protein biochemistry and enables direct visualization of protein dynamics, localization, and interactions in single living cells. Green fluorescent protein (GFP) and its color variants are popular fluorescent tags because they can be genetically fused to any protein of interest with great specificity. 2] However, ...
متن کاملChemical polishing method of GaAs specimens for transmission electron microscopy.
A practical method for transmission electron microscopy specimen preparation of GaAs-based materials with quantum dot structures is presented to show that high-quality image observations in high-resolution transmission electron microscopy (HRTEM) can be effectively obtained. Specimens were prepared in plan-view and cross-section using ion milling, followed by two-steps chemical fine polishing w...
متن کاملIdentification of specimen labeling errors in pathology specimens received from different wards of the hospital: A patient safety approach
Background and Aim: Nowadays, patient safety culture is recognized as an important issue in providing high quality services for the patients around the world. Unsafe care and services can lead to mortality, disability, prolonged hospitalization and increased costs of treatment. Therefore, the present study aimed to identify the errors associated with the labels of pathology samples in Qazvin ho...
متن کاملChemical labeling and enrichment of nitrotyrosine-containing peptides.
Protein tyrosine nitration (PTN) is a post-translational modification of proteins associated with a number of inflammatory diseases. While PTN is rather selective (not all proteins are modified and within a protein, only certain tyrosines are subject to nitration), no consensus sequence has been identified. Since PTN is a low-abundance post-translational modification, it is necessary to enrich ...
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ژورنال
عنوان ژورنال: Science
سال: 1915
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.42.1088.656